Ce mois-ci, la SFI et le Club Français des Jeunes Immunologistes mettent en avant l’article publié dans Nature Immunology, du Dr. Abbas, doctorant dans l’équipe du Dr Marc Dalod, au CIML, Marseille. L’article sera publié sur le site de la SFI durant tout le mois d’avril et concourra pour le prix du ” Meilleur article doctorant” au mois d’octobre prochain.
|Journal||Nat Immunol. 2020 Sep 1; 21(9): 983–997.|
|Titre||The activation trajectory of plasmacytoid dendritic cells in vivo during a viral infection|
|Auteurs||Abdenour Abbas*, Thien-Phong Vu Manh*, Michael Valente, Nils Collinet, Noudjoud Attaf, Chuang Dong, Karima Naciri, Rabie Chelbi, Geoffray Brelurut, Inaki Cervera-Marzal, Benjamin Rauwel, Jean-Luc Davignon, Gilles Bessou, Morgane Thomas-Chollier, Denis Thieffry, Alexandra-Chloé Villani, Pierre Milpied, Marc Dalod**, Elena Tomasello**. *Co-1st; **Co-senior.|
|Résumé||Plasmacytoid dendritic cells (pDCs) are a major source of type I interferon (IFN-I). What other functions pDCs exert in vivo during viral infections is controversial, and more studies are needed to understand their orchestration. In the present study, we characterize in depth and link pDC activation states in animals infected by mouse cytomegalovirus by combining Ifnb1 reporter mice with flow cytometry, single-cell RNA sequencing, confocal microscopy and a cognate CD4 T cell activation assay. We show that IFN-I production and T cell activation were performed by the same pDC, but these occurred sequentially in time and in different micro-anatomical locations. In addition, we show that pDC commitment to IFN-I production was marked early on by their downregulation of leukemia inhibitory factor receptor and was promoted by cell-intrinsic tumor necrosis factor signaling. We propose a new model for how individual pDCs are endowed to exert different functions in vivo during a viral infection, in a manner tightly orchestrated in time and space.|