Spatial, functional and physical analysis of the fibroblast/macrophage interplay in lung cancer
Specific fibroblasts and macrophages favor an immunosuppressive environment across multiple cancer types, limiting the access and activity of tumor-specific killer T cells. The impact of the cellular and molecular dialogue between fibroblasts and macrophages on solid tumor growth, as well as the impact of the tumor microenvironment on this crosstalk, remain largely unexplored.
Our objective is to thoroughly analyze this dialogue in lung cancer to identify key molecular targets and pathways for the development of novel therapeutic strategies.
The project is based on interdisciplinary approaches combining analyses :
– At the spatiotemporal and cellular level, by characterizing samples from a retrospective cohort of patients by multiplex imaging
– At the cellular and molecular level, using our 3D tumor spheroid culture system and real time and confocal microscopy
– At the physical level, by adapting our previous mathematical modeling of the evolution of tumor spheroids
The Lab (http://ow.ly/WjoV50yVbOQ)
Our team is part of the Immunology department (INSERM U932) headed by Dr Ana-Maria Lennon located in the exciting professional environment of Institut Curie, one of the largest European institutions for cancer and biomedical research with strong interdisciplinary traditions.
Dr. Philippe Benaroch’s team is focused on how myeloid cells crosstalk with viruses and tumor cells, and how this interplay is regulated at the molecular level. The project is funded for at least two years and will be performed in collaboration with the team of Dr. Hélène Salmon focusing on fibroblasts and the one of Jean-François Joanny who developed the mathematical modeling.
Qualification and skills
– PhD in Immunology or Cell Biology
– Expertise with immunochemistry and flow cytometry appreciated
– High motivation, scientific rigor, team spirit, proficient in English, and dynamic personality
– International applicants are welcome
M Casanova-Acebes,/…/ P Benaroch, JA Aguirre-Ghiso & M Merad. Lineage tracing reveals the pro-tumorigenic niche role of tissue resident macrophages in early lung cancer lesions. Nature 2021. doi:10.1038/s41586-021-03651-8
Cannac M, Nikolic J & P Benaroch. Cancer immunotherapies based on genetically engineered macrophages. Cancer Immunology Research 2022 In press
Grout JA, et al. Salmon H. Spatial positioning and matrix programs of cancer-associated fibroblasts promote T cell exclusion in human lung tumors. BIORXIV 2022. In final revisions in Cancer Discovery
Adar RM, Joanny JF. Permeation Instabilities in Active Polar Gels. Phys Rev Lett. 2021. 27(18):188001
Posted July 2022, until candidate is selectedDownload details